microtiter plate lid, black (corning, # 3935) (Corning Life Sciences)
90
Structured Review
Corning Life Sciences
microtiter plate lid, black (corning, # 3935)
Microtiter Plate Lid, Black (Corning, # 3935), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microtiter+plate+lid%2C+black/96+well+plates/pmc03321558-398-46-52
Average 90 stars, based on 1 article reviews
Microtiter Plate Lid, Black (Corning, # 3935), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microtiter+plate+lid%2C+black/96+well+plates/pmc03321558-398-46-52
Average 90 stars, based on 1 article reviews
microtiter plate lid, black (corning, # 3935) - by Bioz Stars,
2026-09
90/100 stars
Images
Related Articles
other:Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: CodeLink Activated Slides: GE Healthcare (Amersham-300011), Piscataway, NJ 20X SSC Sigma S6639-1L 5M Sodium Chloride, Sigma S5150-1L Bicine, Sigma B8660-1KG Taurine, Sigma T8691-100G Fluorescein, Sigma F-6377 Anhydrous monobasic sodium phosphate, Sigma S-3139 Dibasic sodium phosphate, heptahydrate, Sigma S-9390 6.2 Sample Preparation (refer to point 5.2 of Discussion of Equipment) 6.2.a Reagents 0.5 pmol/μl 3′ NNNNNNNN- (dA) 12 T (biotin) (dA) 12 -Biotin 5′ Oligonucleotide primer 5X First-strand buffer 0.1 M Dithiothreitol (DTT) 10 mM dNTP mix SuperscriptTM II RNaseH − reverse transcriptase (200 U/μl) (Invitrogen 18064-014) 10mM dNTP mix (Invitrogen 18427-013) 6.2.b Equipment and Materials Supplied by User Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes (10, 20, 200, 1000 μl) Nanodrop UV spectrophotometer Microcentrifuge, room temperature and 4°C Water bath (settings 70°C, 65°C, 37°C) Sterile, Nuclease-Free conical tubes (15 and 50 ml) Speed-Vac Concentrator Vortex Pipet Aid and disposable pipets 6.3 miRNA Microarray Hybridization and Data Collection (refer to point 5.3 of Discussion of Equipment) 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) Microarray:Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free gloves Microcentrifuge Microtiter plate lid, Black (Corning, # 3935) Bioarray Processors (GE, Healthcare) Bioarray Rack (GE No. 600010) Small Reagent Reservoir (GE No. 600011) Large Reagent Reservoir (GE No. 600013) Bioarray Removal Tool (GE No. 600015) Bioarray Position Tool (GE No. 600016) 6.3.2 miRNA Microarray Post-Hybridization 6.3.2a Stock Solutions for Post-hybridization array processing TNT Buffer (20 L) 0.1 M Tris-HCl, pH 7.6 0.15 M NaCl 0.05% Tween-20 Rinse a 25 L Carboy out with 150 ml of isopropanol. .. 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free Hybridization:Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free gloves Microcentrifuge Microtiter plate lid, Black (Corning, # 3935) Bioarray Processors (GE, Healthcare) Bioarray Rack (GE No. 600010) Small Reagent Reservoir (GE No. 600011) Large Reagent Reservoir (GE No. 600013) Bioarray Removal Tool (GE No. 600015) Bioarray Position Tool (GE No. 600016) 6.3.2 miRNA Microarray Post-Hybridization 6.3.2a Stock Solutions for Post-hybridization array processing TNT Buffer (20 L) 0.1 M Tris-HCl, pH 7.6 0.15 M NaCl 0.05% Tween-20 Rinse a 25 L Carboy out with 150 ml of isopropanol. .. 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free Blocking Assay:Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free gloves Microcentrifuge Microtiter plate lid, Black (Corning, # 3935) Bioarray Processors (GE, Healthcare) Bioarray Rack (GE No. 600010) Small Reagent Reservoir (GE No. 600011) Large Reagent Reservoir (GE No. 600013) Bioarray Removal Tool (GE No. 600015) Bioarray Position Tool (GE No. 600016) 6.3.2 miRNA Microarray Post-Hybridization 6.3.2a Stock Solutions for Post-hybridization array processing TNT Buffer (20 L) 0.1 M Tris-HCl, pH 7.6 0.15 M NaCl 0.05% Tween-20 Rinse a 25 L Carboy out with 150 ml of isopropanol. .. 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free Staining:Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free gloves Microcentrifuge Microtiter plate lid, Black (Corning, # 3935) Bioarray Processors (GE, Healthcare) Bioarray Rack (GE No. 600010) Small Reagent Reservoir (GE No. 600011) Large Reagent Reservoir (GE No. 600013) Bioarray Removal Tool (GE No. 600015) Bioarray Position Tool (GE No. 600016) 6.3.2 miRNA Microarray Post-Hybridization 6.3.2a Stock Solutions for Post-hybridization array processing TNT Buffer (20 L) 0.1 M Tris-HCl, pH 7.6 0.15 M NaCl 0.05% Tween-20 Rinse a 25 L Carboy out with 150 ml of isopropanol. .. 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free Transferring:Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free gloves Microcentrifuge Microtiter plate lid, Black (Corning, # 3935) Bioarray Processors (GE, Healthcare) Bioarray Rack (GE No. 600010) Small Reagent Reservoir (GE No. 600011) Large Reagent Reservoir (GE No. 600013) Bioarray Removal Tool (GE No. 600015) Bioarray Position Tool (GE No. 600016) 6.3.2 miRNA Microarray Post-Hybridization 6.3.2a Stock Solutions for Post-hybridization array processing TNT Buffer (20 L) 0.1 M Tris-HCl, pH 7.6 0.15 M NaCl 0.05% Tween-20 Rinse a 25 L Carboy out with 150 ml of isopropanol. .. 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free Sterility:Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free gloves Microcentrifuge Microtiter plate lid, Black (Corning, # 3935) Bioarray Processors (GE, Healthcare) Bioarray Rack (GE No. 600010) Small Reagent Reservoir (GE No. 600011) Large Reagent Reservoir (GE No. 600013) Bioarray Removal Tool (GE No. 600015) Bioarray Position Tool (GE No. 600016) 6.3.2 miRNA Microarray Post-Hybridization 6.3.2a Stock Solutions for Post-hybridization array processing TNT Buffer (20 L) 0.1 M Tris-HCl, pH 7.6 0.15 M NaCl 0.05% Tween-20 Rinse a 25 L Carboy out with 150 ml of isopropanol. .. 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free Aerosol:Article Title: Expression profiling of microRNA using oligo DNA arrays Article Snippet: 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free gloves Microcentrifuge Microtiter plate lid, Black (Corning, # 3935) Bioarray Processors (GE, Healthcare) Bioarray Rack (GE No. 600010) Small Reagent Reservoir (GE No. 600011) Large Reagent Reservoir (GE No. 600013) Bioarray Removal Tool (GE No. 600015) Bioarray Position Tool (GE No. 600016) 6.3.2 miRNA Microarray Post-Hybridization 6.3.2a Stock Solutions for Post-hybridization array processing TNT Buffer (20 L) 0.1 M Tris-HCl, pH 7.6 0.15 M NaCl 0.05% Tween-20 Rinse a 25 L Carboy out with 150 ml of isopropanol. .. 6.3.1 miRNA Microarray Hybridization 6.3.1a Required Reagents/Kits Supplied by User 0.5% NEN Blocking Reagent (Perkin Elmer No. FP1020) Molecular Probes Streptavidin-Alexa Fluor® 647 conjugate (staining solution is a 1:500 dilution in TNB; see Appendix 1) (Molecular Probes No. S-21374) Nuclease-free H 2 O (Ambion No. 9915G) 1 X PBS; pH 7.4 (Invitrogen/LTI No. 10010-023) 1M Tris-HCl, pH 7.6 (Sigma No. T-2788) 5M NaCl (Sigma No. S-5150) Tween ® -20 (Sigma No. P-7949) Formamide (Sigma) 50X Denhardt Solution (Sigma) 6.3.1b Other Equipment and Materials Supplied by User Tecan HS 4800 Hybridization Station Axon GenePix 4000B scanner Computer configured for Axon 4000B Scanner New Brunswick InnovaTM 4080 shaking incubator Sigma/Qiagen Centrifuge (4°–15°C) (Qiagen No. 81010) Centrifuge Plate Rotor-2x96 (Qiagen No.81031) Pipette tips, sterile, RNase-free, and aerosol-resistant Microcentrifuge tubes, sterile, RNase-free, 1.7 ml Micropipettes Powder-free |